Elucidating organ-specific metabolic toxicity chemistry from electrochemiluminescent enzyme/DNA arrays and bioreactor bead-LC-MS/MS† †Electronic supplementary information (ESI) available: Experimental details, 9 additional figures and 5 tables documenting system characterization, and raw array and LC-MS/MS results. See DOI: 10.1039/c4sc03401e Click here for additional data file.

[Ru(bpy)2(PVP)10] (RuPVP (bpy = 2,2-bipyridyl; PVP = poly(4-vinylpyridine)) was synthesized and characterized as described previously. Styrene (MW= 104.15), 2Acetylaminofluorene (2-AAF, MW= 223.27), 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK, MW= 207.23), poly(diallyldimethylammonium chloride) (PDDA, average Mw= 100,000200,000), poly(sodium 4-styrenesulfonate) (PSS, average MW= 70000), calf thymus DNA (Type I) and all other chemicals were from Sigma. Pooled male human liver microsomes (Liver, 20 mg mL in 250 mM sucrose) contained (a) 20 mg mL total protein content , (b) total cyt P450 content of 340 pmol mg of protein using the method of Omura and Sato, baculovirus-insect cell expressed cyt P450 1B1 supersomes (cyt P450 1B1), 4.5 mg/ml in 100mM potassium phosphate buffer of pH 7.4 with representative total cyt P450 content of 220 pmol mg of protein; baculovirus-insect cell expressed cyt P450 1A1 supersomes (cyt P450 1A1), 5.0 mg/ml in 100mM potassium phosphate buffer of pH 7.4 with representative total cyt P450 content of 120 pmol mg of protein; baculovirus-insect cell expressed cyt P450 3A4 supersomes (cyt P450 3A4), 5.0 mg/ml in 100mM potassium phosphate buffer of pH 7.4 with representative total cyt P450 content of 200 pmol mg of protein; and baculovirus-insect cell expressed cyt P450 3A5 supersomes (cyt P450 3A5), 14 mg/ml in 100mM potassium phosphate buffer of pH 7.4 with representative total cyt P450 content of 1000 pmol mg of protein; were from BD Gentest (Woburn, MA). Human lung microsomes (Lung), 10 mg mL in 250 mM sucrose; Human intestinal microsomes (Intestine), 20 mg mL in 250 mM sucrose; Human kidney microsomes (Kidney), 10 mg mL in 250 mM sucrose; Human liver cytosol (HLC), 20 mg mL in 50 mM Tris 150 mM KCl, 2 mM EDTA of pH 7.5; Human lung cytosol (HLuC), 12.1 mg mL in 250 mM sucrose; Human intestinal cytosol (HIC),11.7 mg mL in 250 mM sucrose; Human kidney cytosol (HKC), 10.6 mg mL in 250 mM sucrose were purchased from Celsis (Chicago, IL).